Naslov
Disruption of the putative cyclase, otcD1, and methylase, otcZ, genes of the oxytetracycline gene cluster of Streptomyces rimosus

Autori
Petković, Hrvoje ; Zhou, Lihong ; Waterman, G. Peter ; Hranueli, Daslav ; Raspor, Peter ; Hunter, S. Iain

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th European Congress on Biotechnology : Book of Abstracts / László, N. - Budimpešta : Hungarian Biochemical Society, 1997, 220-221 (TU2517)

Skup
8th European Congress on Biotechnology

Mjesto i datum
Budimpešta, Mađarska, 17.08.1997. - 21.08.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Streptomyces; oxytetracycline; cyclase; methylase

Sažetak
The entire gene cluster for oxytetracycline (OTC) biosynthesis has been cloned (1) from S. rimosus. A strategy to deduce the function of each gene is to identify the metabolite(s) made by a recombinant which has the gene disrupted, e.g. (2). Gene disruption of a putative cyclase gene, otcD1, was undertaken within the chromosome of S. rimosus. OtcD1 is thought to be involved in the folding, cyclization and aromatization of the hypothetical nonaketide intermediate in OTC biosynthesis. The same procedure was also completed for otcZ , which is thought to methylate either the C-4 or C-6 position of the OTC backbone. However, in this case, the otc cluster was present on low copy number plasmid pIJ941 in S. lividans TK64. The disrupted strains no longer produced OTC. Whereas the strain with the disrupted otcZ gene produced one new compound, the mutant with the disrupted otcD1 gene produced a number of compounds, not detectable in the wild-type strain. Preliminary results showed that those isolated are of polyketide structure. The construction of these mutants and further analysis of the metabolites that they make will be presented. 1. Binnie, C., M. Warren & M. Butler. J. Bacteriol. 171: 887-895, 1989. 2. Khosla, C., S. Ebert-Khosla & D.A. Hopwood. Mol. Microbiol. 21: 3237-3249, 1992.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA