Pregled bibliografske jedinice broj: 14195
Genetic instability affecting the terminal regions of Streptomyces chromosomes
Genetic instability affecting the terminal regions of Streptomyces chromosomes // Xth International Symposium on Biology of Actinomycetes, ISBA '97 : Abstract Book / Chiao, J. (ur.).
Peking, Kina: Chinese Society for Microbiology, 1997. (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 14195 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Genetic instability affecting the terminal regions of Streptomyces chromosomes
Autori
Cullum, John ; Pandža, Kenan ; Pandža, Suada ; Biuković, Goran ; Redenbach, Matthias ; Denapaite, Dalia ; Hranueli, Daslav
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Xth International Symposium on Biology of Actinomycetes, ISBA '97 : Abstract Book
/ Chiao, J. - : Chinese Society for Microbiology, 1997
Skup
Xth International Symposium on Biology of Actinomycetes
Mjesto i datum
Peking, Kina, 27.05.1997. - 30.05.1997
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Streptomyces; genetic instability; linear chromosome; linear plasmid
Sažetak
Spontaneous mutants of S. rimosus were characterised which showed changes in the 387 kb linear plasmid pPZG101 (Gravius et al., 1994). In one case an end of pPZG101 had integrated into the linear chromosome which resulted in the replacement of one chromosome end with a plasmid end. A second mutant carried a 1 Mb plasmid-prime in which one end of the linear plasmid had been replaced by a chromosome end. This resulted in a plasmid (pPZG103) carrying the oxytetracycline synthesis cluster which lies 600 kb from the chromosome end. In S. lividans 66 mutants were observed that had lost one or both chromosome ends. Mutants that had lost both chromosome ends had also lost the linear plasmid SLP2 (Lin et al., 1993), which has one end identical to the chromosome ends. SLP2 could not be reintroduced into such mutants. This suggests the existence of chromosomal genes in the deleted regions that are needed for stable replication of linear ends. Gravius B, Glocker D, Pigac J, Pandza K, Hranueli D, Cullum J (1994) The 387 kb linear plasmid pPZG101 of Streptomyces rimosus and its interactions with the chromosome. Microbiology, 140, 2271-2277. Lin YS, Kieser HM, Hopwood DA, Chen CW (1993) The chromosomal DNA of Streptomyces lividans 66 is linear. Mol Microbiol, 10, 923-933.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija
