Naslov
Biološka razgradnja amonijaka u kemijski definiranim medijima
(Biological Ammonia Degradation in Chemically Defined Basis)

Autori
Tomerlin, Ljiljanka ; Has-Schön, Elizabeta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka / - Primošten, 1998

Skup
Međunarodna konferencija o zaštiti voda u nacionalnim parkovima i drugim zaštićenim područjima

Mjesto i datum
Primošten, Hrvatska, 20.05.1998. - 23.05.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
amonijak; biološka razgradnja; kemijski definirani medij
(ammonia; biological degradation; chemically defined basis)

Sažetak
In our previous investigation, we showed that associated microbial population (AMP), composed of four bacterial (Pseudomonas sp1 and sp2, Flavobacterium and Corynebacterium) and one yeast (Trichosporon) strains is capable to eliminate as much as 60 mg/L NO3-N from chemically defined basis (CDB) used for their aerobic cultivation, under appropriate conditions. Moreover, NO2 and NH4 nitrogen forms showed no tendency to accumulate as the consequence of denitrification, indicating also the nitrification potentials of AMP. Therefore, we continued our research cultivating AMP in CDB containing high ammonia concentrations (20, 60 and 100 mg/L NH4-N), to find out if an excessive biodegradation of this nitrogen is possible. AMP has been prepared by multiple biomass transfer between the solid and liquid chemically defined basis, as already described. To a very poor CDB, containing only phosphates and Mg2+, different carbon compounds, used as electron donors, were added: glucose (C-1), Na3-citrate (C_2), Na-acetate (C-3) and methanol (C-4). Experiments were performed at two starting pH (5, 5 and 7, 5), which were corrected to the initial value after each experimental phase. AMP growth in CDB took place 8 and 18 hours (20 mg/L NH4) or 24 and 48 hours (60 and 100 mg/L NH4). NH4, NO3 and NO2-N concentrations were determined at the beginning of experiment and at the end of each experimental step. The results obtained prove that AMP can efficiently (almost 100%) eliminate as high as 100 mg/L NH4 from the cultivation medium, if adequate electron donors and pH values are applied. Glucose, Na3-citrate and Na-acetate display similar stimulatory effects on AMP activation, unlike methanol (NH4 concentration did not change at the end of experiment, compared to the initial values). For higher ammonia concentrations, better results are obtained at higher pH value. Finally, we conclude that aerobic AMP growth in CDB performs simultaneous nitrification/denitrification activity, stimulated by the presence of appropriate carbon compounds, used as electron donors. Consequently, initial ammonia concentrations ranging from 20 to 100 mg/L can be completely removed from water solutions used for AMP growth, without concomitant NO3 or NO2 accumulation. Therefore, we consider that AMP or similar heterogeneous microbial mixed populations might be a powerful and economical tool for ammonia elimination from different intoxicated water milieus.

Izvorni jezik
Engleski

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