Pregled bibliografske jedinice broj: 139128
Human first trimester decidua macrophages display phenotype of alternative activation
Human first trimester decidua macrophages display phenotype of alternative activation // Keystone Symposia, 2003 Abstract Book / Mosser M., David ; Karp L., Christopher ; Gordon, Siamon (ur.).
Taos: -, 2003. str. 72-72 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 139128 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Human first trimester decidua macrophages display phenotype of alternative activation
Autori
Sedlmayr, Peter ; Ćupurdija, Kristijan ; Azzola, Dagmar ; Morales, Pedro ; Hunt S., Joan ; Wintersteiger, Reinhold ; Goerdt, Sergij ; Dohr, Gottfried
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Keystone Symposia, 2003 Abstract Book
/ Mosser M., David ; Karp L., Christopher ; Gordon, Siamon - Taos, 2003, 72-72
Skup
Keystone Symposia, Regulatory and Effector Functions of Macrophages
Mjesto i datum
Taos (NM), Sjedinjene Američke DRžave, 30.01.2003. - 04.02.2003
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
-
Sažetak
"Classical" activation of macrophages, induced by IFNgama and LPS leads to secretion of proinflammatory cytokines and support of Th1-driven immune reactions which may be detrimental to early pregnancy, where macrophages make up for up to 30% of bone marrow derived cells in the human decidua during the first trimenon. In contrast, "alternally activated" macrophages (AAM) induced e.g. by IL-4 and glucocorticoids, IL-10 or IL-13, display an immunosuppressive effect towards Th1 mediated immune responses, which may relate to successful pregnancy. AAM are characterized by a distinctive molecular repertoire. The purpose of our study was to study the activation-type related phenotype of DM on the basis of expression of the following markers: MS-1 high molecular weight protein, fibronectin, coagulation factor XIIIa, IL-10 (which are characteristic for AAM) and iNOS (a marker of classical activation). The methods used included immunoperoxidase staining of cryocut sections, double fluorescence flow cytometry of permeabilized cells isolated from first trimester decidua, confocal laser scanning cytometry and (in the case of IL-10 and iNOS) RT-PCR of flow sorted DM. On the protein level we found expression of IL-10, MS-1, fibronectin and F XIIIa. On the mRNA level, IL-10 was detectable in contrast to iNOS, which was negative. Our data provede a phenotypic pattern characteristic of AAM. Functional data are required to complement these studies.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
