Naslov
Profile of Cytokines in Aqueous Humor from Corneal Graft Recipients

Autori
Dekaris I, Gabrić N, Mažuran R, Karaman-Martinović Ž, Mravičić I

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book of First SERI-ARVO meeting on research in vision and Ophtalmology / - , 2003

Skup
First SERI-ARVO meeting on research in vision and Ophtalmology, Singapore

Mjesto i datum
Singapur, 06.02.2003. - 09.02.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cytokine profile; aqueous humor; corneal transplantation

Sažetak
PURPOSE: To determine cytokine profile in aqueous humor (AqH) of patients undergoing corneal transplantation, and to establish whether cytokine profile at the time of surgery influenced corneal graft outcome. METHODS: Proinflammatory (TNF-&#946; and IFN-&#947; ) and immunosuppressive (TGF-&#946; 2) cytokine levels were measured in AqH and serum of 44 patients with either non-inflammatory diseases: keratoconus (n=8), bullous keratopathy (n=7), stromal dystrophy (n=3) or inflammatory diseases: corneal scars (n=10), graft rejection (n=5), pending perforation (n=4), chemical burns (n=4), keratitis/uveitis (n=1), infectious keratitis (n=1), perforated ulcer (n=1). Control AqH and sera were from cadavers having corneas without pathology. RESULTS: TGF-&#946; 2 concentration in the AqH of patients with non-inflammatory corneal diseases was similar to controls (2, 605 &plusmn ; ; 204 pg/mL vs 2, 200 &plusmn ; ; 100 pg/mL). In inflammatory corneal diseases, TGF-&#946; 2 in AqH was significantly lower (1, 400 &plusmn ; ; 375 pg/mL, p<0.001). TNF-&#946; was detected in the AqH of 16 out of 26 patients with inflammatory corneal diseases and in all patients with stromal dystrophies, but was undetectable in cases of keratoconus and bullous keratopathy. Low levels of IFN-&#947; were present in all AqH samples. Patients&#8217; sera contained significantly less cytokine (up to 252 pg/mL) then their AqH (p<0.001). We have set an arbitrary cut-off point for TGF-&#946; 2 level in AqH at 1, 500 pg/mL and divided all investigated samples (44 patients + 10 controls) into two groups, one with high and the other with low TGF-&#946; 2 concentration. The coefficient of contingency showed that patients with high TGF-&#946; 2 concentration in their AqH had significantly greater chance for graft acceptance than those with low TGF-&#946; 2 concentration (p<0.001). CONCLUSION: High TGF-&#946; 2 concentrations in the eyes without intraocular inflammation suggest its immunosuppressive role in human eyes. High concentration of TGF-&#946; 2 (>1, 500 pg/mL) was associated with graft acceptance. Also, absence of proinflammatory TNF-&#946; in AqH increased the graft acceptance rate.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA