Pregled bibliografske jedinice broj: 138094
Potentiometric and amperometric determination of cyanide in alcohol beverages
Potentiometric and amperometric determination of cyanide in alcohol beverages // XVIII. hrvatski skup kemičara i kemijskih inženjera / Zrnčević, Stanka (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2003. (poster, domaća recenzija, sažetak, znanstveni)
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Naslov
Potentiometric and amperometric determination of cyanide in alcohol beverages
Autori
Mikšaj, Miroslav ; Filipović-Kovačević, Željka ; Kruhak, Ivan ; Šalamon, Dean
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
XVIII. hrvatski skup kemičara i kemijskih inženjera
/ Zrnčević, Stanka - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2003
Skup
XVIII. hrvatski skup kemičara i kemijskih inženjera
Mjesto i datum
Zagreb, Hrvatska, 16.02.2003. - 19.02.2003
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
cyanides; potentiometric sensor; amperometric sensor; saccharomyces cerevisiae
Sažetak
It is well known that the toxicity of cyanide is due to inhibition of respiration activity of yeasts by its binding to cytochrome oxidase complexes. These phenomena can be used for development of a cyanide biosensor consisting of oxygen electrode with immobilised S. cerevisiae. The cyanide present in beverage samples inhibits the electron chain reaction of respiration system in mitochondria decreasing the respiration activity. Fruit brandies in which cyanide contents were determined by potentiometry were used for determination of total respiration activity of Saccharomyces cerevisiae. The comparison of two methods (ISE-potentiometry and measurement by amperometric biosensor) for the determination of cyanide was investigated. Effect of cyanide concentration on microbial cells was observed by measuring the decrease of the current until a steady-state was reached. When a steady-state current was reached, the microbial electrode was immersed in pure growth medium to recover. The recovery depends on the cyanide concentration in brandy present in growth medium. The correlation between results obtained by two methods is good, although the cyanide concentrations obtained by ISE-potentiometry seemed to be slightly lower than those obtained by amperometric microbial biosensor.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
