Naslov
Tumorski makrofagi u regulaciji angiogeneze invazivnog duktalnog raka dojke

Autori
Valković, Toni

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Medicinski fakultet

Mjesto
Rijeka

Datum
17.09

Godina
2002

Stranica
124

Mentor
Jonjić, Nives

Neposredni voditelj
Jonjić, Nives

Ključne riječi
duktalni invazivni karcinom dojke NOS; tumorski makrofagi; gustoća malih krvnih žila; monocitna kemotaktična bjelančevina -1; vaskularni endotelni čimbenik rasta; bazični fibroblastni čimbenik rasta; angiogeneza; kemotaksija
(Ductal Invasive Breast Cancer NOS; Tumor Associacted Macrophages; Microvessel Density; Monocyte Chemotactic Protein -1; Vascular Endothelial Growth Factor; Basic Fibroblast Growth Factor; Angiogenesis; Chemotaxis)

Sažetak
Objectives: the aims of this investigation were to determine infiltrations of Tumor Associated Machrophages (TAM) as well as expresion of Vascular Endothelial Growth Factor (VEGF) and basic Fibroblast Growth Factor (bFGF) in the tumr parenchymal of ductal invasive breast carcinomas NOS and correlate these parameters to each other and wth maximal microvessel density (MVD). Also, the expresion of Monocite Chemotactic Protein - 1 (MCP - 1) in the tumr cells of this kind of breast cancer was determined and compared with macrophage infiltrations and above - mentioned parameters. Finally, the tumor´s capability to produce mentioned chemokine was, also, analysed by determination the level of messenger - ribonucleic acid (mRNA) for MCP - 1. Material and methods: 97 ductal invasive breast carcinomas NOS were analysed by immunohistochemistry using "avidin - biotin" method and macrophage infiltration, MVD, expression of MCP - 1, VEGF and FGF (63 samples of breast carcinoma were analysed) in the tumor cells were determined. The expression of messenger - ribonucleic acid for MCP - 1 was examined by reverse transcriptase - polymerase chain reaction (RT - PCR) in the 8 samples of breast carcinomas and control normal breast tissues. Statistical analysis was performed by the personal computer using chi square test, t - test and "one way" analysis of variance. Results: statistical analysis pointed that breast carcinomas with only indivudual stromal macrophages had significantly decreased MVD in the comparation with focally and diffusely infiltrated tumros (p = 0, 005). Also, postive corelations between parenchymal expression of VEGF and MVD (p < 0, 001), macrophage infiltrations (p = 0, 003) and expression of bFGF (p = 0, 016) were found. The corelation between expession of MCP - 1 and macrophages infiltrations was not found and the results of the polymerase chain reaction pointed that breast carcinomas, as well as normal breast tissue can expressed messenger ribonucleic acid for MCP - 1. Conclusion: in the ductal invasive breast carcinomas NOS, TAM promote angiogenesis as well as VEGF produced by tumor parenchymal. Also, this angiogenic factor has chemotactic influence on macrophages. bFGF has no angiogenic properties in this kind of breast cancer and the expression of this angiogenic factor and VEGF can be achieved by the same regulatory mechanisms.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA