Naslov
Rosmarinic acid synthesis in transgenic callus of Coleus blumei Benth.

Autori
Bauer, Nataša ; Leljak-Levanić, Dunja ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th Congress of the Federation of Europeaan Societies of Plant Physology - Book of Abstracts / Roubelakis-Angelakis, Kalliopi A. - Lahti : FESPP, 2002

Skup
Congress of the Federation of European Societies of Plant Physiology

Mjesto i datum
Heraklion, Grčka, 02.10.2002. - 06.10.2002

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Coleus blumei; Rosmarinic acid; Agrobacterium

Sažetak
Rosmarinic acid (RA) is a natural antioxidant found especially in plants of Lamiaceae and several Boraginaceae species. Coleus blumei (Lamiaceae) is well known to produce significant amount of RA. After leaf transformation of three genotypes of Coleus blumei, using 6 wild and mutant strains of Agrobacterium spp., 16 transgenic callus lines and one hairy root line were established. Among them, two lines (Ac1 and Ac2), induced on the red colored leaves with the wild strain Agrobacterium tumefaciens A281 were compared mutually. Both lines grew well on hormone-free MS medium. The lines differed in tissue color and structure, growth rate and rosmarinic acid (RA) production. After 36 days, the growth index on basal MS medium of lines Ac1 and Ac2 were 12.9 and 30, respectively. On average the line Ac1 accumulated 7.49 mg RA and the line Ac2 4.45 mg RA per tube. The growth and RA production of the lines could be modified by the change of the medium composition. Different concentrations of sucrose (30 g/L, 40 g/L, 50 g/L and 70 g/L), presence of phenylalanine (0.1 g/L, 0.3 g/L and 1 g/L) and reduction of ammonium and nitrate ion concentrations in basal MS medium influenced the growth and production of RA. The line Ac1 produced the highest RA amount (7.09 mg of RA per tube) on medium with reduced ammonium and nitrate ion concentrations, while the addition of phenylalanine in nutrient medium was the most efficient for RA production in callus line Ac2 (6.59 mg of RA per tube). Two lines responded differently to the same medium composition, but transgenic callus showed the high potential of RA synthesis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA