Naslov
Extracellular glycoproteins have links with callose deposition in embryogenic culture of Cucurbita pepo l.

Autori
Leljak-Levanić, Dunja ; Bauer, Nataša ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Slovenian Symposium on Plant Physiology

Mjesto i datum
Ljubljana, Slovenija, 25.09.2002. - 27.09.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
callose; extracellular glycoproteins; somatic embryogenesis; pumpkin

Sažetak
Extracellular glycoproteins are known to play an important role in development of somatic embryos. On the other side there are data that callosic walls and callose deposition have a role in the first steps of dedifferentiation and initiation of embryogenesis. We analyzed these phenomena in three embryogenic tissue lines of pumpkin that differ in the initiation and maintained growth conditions. Embryos of three lines developed similarly on the media with the same composition of nitrogen salts, auxin and tunicamycin. Cells of tissue lines grown in NH4Cl-containing MS media had a high callose content but protein excretion and embryo maturation were inhibited. After the transfer of the tissue to MSC medium with conventional nitrogen, callose content decreased, followed by secretion of 34, 37.5 and 68 kDa glycoproteins specific for embryo maturation. Secretion of some glycoproteins depended on the presence of 2, 4-D, or were specific to embryogenic line that was induced in a medium with 2, 4-D. Tunicamycin did not prevent embryogenesis, but it did have an impact on the glycosylation of proteins and embryo maturation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA