Naslov
EXPRESSION OF MULTIXENOBIOTIC RESISTANCE IN EUROPEAN HONEYBEE STRAINS (Apis mellifera L.)

Autori
Dražić, Marica Maja ; Smital, Tvrtko ; Britvić, Smilja ; Krča, Sanja ; Jelaska, Dalibor ; Bubalo, Dragan ; Kezić, Nikola

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programe and Book of Abstract, XXXVIIIth APIMONDIA, International Apicultural Congress, Ljubljana, Slovenia, August 24 - 29. 2003. / Poklukar, Janez ; Kozmus, Peter - Ljubljana : APIMONDIA, 2003, 674-675

Skup
XXXVIIIth APIMONDIA, International Apicultural Congress

Mjesto i datum
Ljubljana, Slovenija, 24.08.2003. - 29.08.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Honeybee strains; Multixenobiotic resistance

Sažetak
Mechanism of multixenobiotic resistance (MXR), similar to multidrug resistance (MDR) in drug resistant tumor cells, has been identified in a broad spectrum of taxons. This mechanism prevents intracellular accumulation and potentially toxic effects equally from endogenous as well as environmental toxins. Its natural activity may be very different in different populations. Different strains of European honeybees (Apis mellifera L.) were kept on the Island of Unije. Colonies were invaded with a high number of varroa mites (Varroa destructor), and kept without any treatment against parasite. We measured the potential of MXR inhibitor cyclosporine A (CA) to increase accumulation rate of a fluorescent model MXR substrate rhodamine B (RB) in the midgut tissues of honeybees previously exposed to high concentrations of RB in the presence or in the absence of MXR inhibitor. We expected to find differences between strains of bees, which may be important in explaining the variation in their susceptibility to fatal outcomes after invasion with Varroa destructor. The influence of inhibitor CA on accumulation of RB in midgut tissue cells varried from 15, 6% in Hybrid bees up to 57, 3% in Buckfast bees. The different levels of MXR activity determined within this study could be a result of evolution or of the selection pressure during the life history of the different honeybee populations. That level might be, at least partly, responsible for the resistance, or for the sensitivity to environmental stress, as well as to the parasite invasion.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA