Naslov
Distribution of apo H dependent autoantibodies and apo H phenotypes among selected patients showing lupus anticoagulant activity

Autori
Vogrinc, Željka ; Trbojević-Čepe, Milica ; Coen, Desiree ; Stavljenić Rukavina, Ana

Izvornik
Clinical chemistry and laboratory medicine (1434-6621) 41 (2003);

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
Apo H phenotypes; Lupus anticoagulans; Apo H-dependent autoantibodies

Sažetak
It has been found that apo H (ß-2-glycoprotein I) is composed of five suchi domains (I-V) and its genetic polymorphism was reported. Apo H is also the target antigen for binding of certain "antiphospholipid" antibodies. It has been reported that antibodies directed against III domain and IV domain also exert lupus anticoagulant activity (LAC). The aim of our study was to evaluate the possibility that the presence of less frequent apoH phenotypes, i.e. alleles, may induce structural or conformational changes in these "LAC-dependent" regions that may initiate apo H-dependent/LAC+ type of autoimmune response. We selected patients showing prolonged APTT, confirmed LAC activity and none or low titres of antibodies to "pure" cardiolipin. Many of them had some clinical or serological features of SLE or lupus-like diseases and a high prevalence of clinical manifestation of antiphospholipid syndrome. Antibodies to apoH were determined by commercially available anticardiolipin/apoH ELISA kit. ApoH phenotypes were demonstrated by isoelectric focusing followed by immunoblotting. Our results showed that 79% (64/81) of our selected patients (LAC+) also had elevated apoH-dependent antibody titres. The strong nonlinear correlation (r=0.8595) was observed between LAC activities and the titres of apoH-dependent autoantibodies. In our study we didn't find significant differences between the distribution of apoH phenotypes among the control subjects (n=166) and patients with apoH-dependent/LAC+ autoantibodies.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA