Naslov
Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L.

Autori
Leljak-Levanić, Dunja ; Bauer, Nataša ; Mihaljević, Snježana ; Jelaska, Sibila

Izvornik
Plant Cell Reports (0721-7714) 23 (2004); 120-127

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
5-azacytidine ; Cucurbita pepo L. ; Hormone-free medium ; DNA methylation ; Somatic embryogenesis

Sažetak
Three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2, 4-dichlorophenoxyacetic acid (2, 4-D). Somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2, 4-D, nitrogen sources. The highest rate of DNA methylation was in the early embryo stages, predominantly on MSC medium with 2, 4-D and on auxin-free medium supplemented with 1.0 mM NH4Cl. DNA methylation was correlated with early embryo development in a manner that was not exclusively dependent on the presence/absence of exogenous auxin. DNA methylation decreased during embryo maturation on auxin-free MSC medium and on auxin-free MSC supplemented with 12.3 uM 5- azacytidine (5-azaC). The embryogenic features of the pumpkin tissue were preserved, even after a 2- month treatment with 5-azaC.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA