Naslov
Representational difference analysis reveals DNA differences between Q. robur and Q. suber ; an implication in the study of the genome evolution in Quercus genus

Autori
Zoldoš, Vlatka ; Šiljak-Yakovlev, Sonja ; Papeš, Dražena ; Sarr, A. ; Panaud, Olivier

Izvornik
Molecular Genetics and Genomics (1617-4615) 265 (2001); 234-241

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hybridization in situ; dispersed repetitive DNA; genome evolution; retrotransposons; Quercus

Sažetak
Very similar karyotypes and heterochromatin organization, the identical number/position of ribosomal loci, and very similar genome sizes characterize the common oak (Q. robur) and the cork oak (Q. suber), the two distantly related oak species. Representational Difference Analysis (RDA) was used to subtract the genome of Q. robur against the genome of Q. suber in order to search for genome differentiation. The bank of 400 clones (bearing RDA fragments) that represent genome differences between two species was obtained. Thirteen Q. robur specific DNA sequences were analyzed for their molecular and chromosome organization. All of them belong to the repetitive dispersed component of the genome as revealed by Southern hybridization and in situ hybridization. They are present in Q. robur genome in between 100 and 700 copies, and are distributed throughout the length of almost all chromosomes. Homology search between RDA fragments and sequences contained in data banks revealed similarities of all RDA fragments with known retrotransposons. The RDA fragments were also tested for their presence/absence in the genome of six additional oak species of different phylogenetic groups in order to examine the evolutionary dynamics of these DNA sequences.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA