Naslov
Somatic embryogenesis in pumpkin (Cucurbita pepo L.): Control of somatic embryo development by nitrogen compounds

Autori
Leljak-Levanić, Dunja ; Bauer, Nataša ; Mihaljević, Snježana ; Jelaska, Sibila

Izvornik
Journal of Plant Physiology (0176-1617) 161 (2004); 229-236

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cucurbita pepo; nitrogen; pumpkin; somatic embryogenesis

Sažetak
Embryogenic cultures of pumpkin (Cucurbita pepo L.) were initiated from mechanically wounded mature zygotic embryos on 2, 4-D-containing MS medium, and on hormone-free, semisolid modified MS medium containing NH4Cl as the sole source of nitrogen. The habituated line was derived from the embryogenic tissue induced with 2, 4-D and maintained on medium without growth regulators. Sustained subculturing of the three embryogenic lines on a medium with NH4Cl as the sole source of nitrogen enabled the establishment of highly uniform cultures in which no further development into mature embryo stages occurred. The tissue consisting of proembryogenic globules or globular stage embryos was maintained, without decline, for over six years. Globular embryos proceeded to maturity when a combination of reduced (NH4) and unreduced (NO3) forms of nitrogen was provided in medium. Different nitrogen sources in the medium caused changes of medium pH during subculture in the pH range of 4.0-6.5. The tissue growth and embryo development were blocked on medium with pH adjusted and stabilized at 4.0 or at 3.2.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA