Naslov
Expression of nucleolar protein 1 (Nol1) during embryo development.

Autori
Mitrečić, Dinko ; Malnar, Tajana ; Kostović-Knežević, Ljiljana ; Gajović, Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Croatian Congress on Molecular Life Sciences / - , 2002

Skup
1st Croatian Congress on Molecular Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
mouse; embryo; Nol1; nucleolus

Sažetak
Nucleolar protein 1 was found as a marker of nucleolus in highly proliferated cells. It is present in majority of malignant tissues (lung, breast, prostate, colon, and brain), but also in non-malignant, highly proliferative tissues (PHA treated lymphocytes). High expression of Nol1 in different carcinoma is a marker of poor clinical prognosis. Nol1 expression is regulated during cell cycle: its protein synthesis starts at late G1, and peaks in S phase of cell cycle. In order to investigate expression and function of Nol1 gene, the mouse carrying a gene trap modification of Nol1 gene was produced. Embryonic stem cell genome was modified by insertion of nonhomologous DNA construct carrying selector (neoR) and marker (lacZ). From selected embryonic stem cells, animal carrying the corresponding gene modification was generated. Modified gene is tagged and its expression can be monitored by lacZ activity. Due to inserted construct sequence, function of trapped gene could be partially or totally lost, hence function of the gene can be elucidated. The expression of Nol1 gene during development was determined by whole mount histochemical staining by beta-galactosidase substrate X-gal. Staining was found all over the embryo and in all investigated developmental stages. The strongest staining was found between stages E8.5 and E12.5. Although all tissues expressed Nol1, it was not present in all cells. At the cellular level, majority of staining was found within the nucleolus. In order to get insight in the function of Nol1, intercross of heterozygous mice carrying gene trap insertion was performed. Using the PCR method we demonstrated that homozygous mice were not present among newborn animals. Therefore we assumed that homozygous phenotype is lethal.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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