Naslov
Cytotoxic effects of selected pesticides on BHK (Baby Hamster Kidney) 21 C13 cells

Autori
Kniewald, Jasna ; Čabran, Manuela ; Kmetič, Ivana ; Gaurina-Srček, Višnja ; Kniewald, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology Letters, Supplement 1/135, Abstracts of EUROTOX 2002, 15-18 September 2002, Budapest Convention Center, Budapest, Hungary / Kehrer, J. P. ; Dekant, W. ; Oehme, F. W. ; Menzel, D. B. - Amsterdam : Elsevier, 2002, S37-S38

Skup
EUROTOX 2002

Mjesto i datum
Budimpešta, Mađarska, 15.09.2002. - 18.09.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pesticides; lindane; aldrine; 2.4-D; BHK 21 C13 cell line; cytotoxicity

Sažetak
In order to determine cytotoxic effect of rather common used insecticides lindane (L) and aldrine (A) as well as of herbicide 2, 4-D (D) on the animal cell line growing in suspension, the research has been performed. The safety assessment of chemicals and substances like pesticides and the extrapolation of those data into human risk assessment require a lot of expensive and time costing tests in animal species. In order to reduce the number of experimental animals, different primary cells and cell lines are introduced for acute toxicity assays. In the present research BHK 21 C13 cell line was used as a test system for the determination of L, A and D toxicity. Following parameters are examined: cell viability and number of cells measured by trypane-blue exclusion method in Fuchs-Rosenthal heamocytometer, lyzosomal activity measured by neutral red method and total cell protein content measured by Kenacid blue method. BHK 21 C13 cell line was obtained from the American Type Culture Collection (CCL 10). The cells were maintained at 37°C in an atmosphere of 95% air and 5% CO_2 in Glasgow BHK 21 medium supplemented with 5% fetal bovine serum. The cells were seeded 24 hours before the addition of toxicant, with the initial concentration of 2-2.5 x 10^5 cells/mL/well on the 24-Multiwell plates. L was added at the concentrations of 5 to 120 microg/mL/well, aldrine from 2.5 to 50 mmicrog/mL/well and 2, 4-D from 10 to 250 microg/mL/well. The cytotoxicity was determined after 24, 48 and 72 hours. Cytotoxic effects of the tested toxicants were in correlation with the increasing concentrations. The IC_20, IC_50 and IC_80 were detected from the slope of % of inhibition vs. log dose values. Comparing the effects of tested compounds the rate of toxicity was aldrine>lindane >2.4-D. At the same time we have found that the applied colorimetric methods are acceptable for the quantitative determination of cytotoxic effects with BHK 21 C13 cell line. Such approach can significantly reduce the need of the very expensive experiments with animals.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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