Naslov
Plant regeneration from mesocotyl callus of Hordeum vulgare L.

Autori
Jelaska, Sibila ; Rengel, Zdenko ; Cesar, Vera

Izvornik
Plant Cell Reports (0721-7714) 3 (1984), 4; 125-129

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Hordeum vulgare; callus induction

Sažetak
Callus tissue was induced on barley mesocotyl explants of germinated seven-day-old seedlings on MS medium supplemented with 2, 4-D or 2, 4, 5-T in high concentrations. Two morphologically different tissue cultures were maintained in vitro for a long time: a callus tissue without organogenesis and a culture with high rhizogenic capacity. Shoots and plantlets were generated when the auxin-media induced callus was transferred to medium supplemented with 3 mgrM TIBA. In 62% of cultures, during the first five subcultures, four to twentyeight plants per single mesocotyl were obtained. Some cultures produced shoots even in the 9th subculture, being in culture for nearly 14 months. The largest number of plants obtained per one mesocotyl was forty. Plantlets rooted well on MS with 5.7 mgrM IAA and survived transplantation to soil in high percentages.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA