Naslov
Analysis of short tandem repeats for evaluation of chimerism after allogeneic bone marrow transplantation

Autori
Štingl, Katarina ; Grubić, Zorana ; Čečuk-Jeličić, Esma ; Žunec, Renata ; Serventi, Ranka ; Labar, Boris ; Brkljačić-Kerhin, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Croatian Immunology Society-Annual Meeting 2002 / - , 2002

Skup
Godišnji sastanak Hrvatskog imunološkog društva

Mjesto i datum
Trakošćan, Hrvatska, 22.11.2002. - 24.11.2002

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
BMT; STR; chimerism

Sažetak
Mixed chimerism (MC) is a state in which there is a presence of both recipient and donor hemopoietic cells and it is frequently observed after bone marrow transplantation (BMT). MC is useful in comparison of different conditioning and prophylactic regimens as well as for the assessment of graft-versus host disease (GVHD) and therapies that promote engraftment and graft-versus-leukemia (GVL) effect. In some cases, MC can also be predictive of relapse. For these reasons, it is of high interest to follow the chimeric status of patients. Analysis of the short tandem repeats (STR) loci by polymerase chain reaction (PCR) has proved to be a fast, relatively simple and highly informative method for this purpose. In this study we monitored 15 patients suffering from various hematological disorders. Twelve of them underwent classical conditioning regimen while the remaining three patients were subjected to nonmyeloablative conditioning (minitransplantation). DNA was extracted by salting out method from peripheral blood, which was followed by amplification of six STR loci. Additional four loci were amplified in cases of minitransplantation. Samples were then analysed by electrophoresis in an automated laser fluorescence sequencer (ALFexpress). Alleles were determined with Fragment Manager software. Only one patient had two informative loci while others had three or more. For all donor-recipient pairs there was at least one locus with recipient specific allele. MC was detected in all cases of minitransplantation. Level of recipient cells that was detected in two patients after transplantation gradually decreased until they reached full donor chimerism (FDC). Third patient showed constant MC with high percentage of recipient cells and he is now in relapse. Out of four patients with unrelated donors, two patients showed initial MC that turned to FDC. One patient showed no sign of MC at any period. The fourth patient who suffered from acute lymphoblastic leukemia (ALL) showed FDC until 160th day post BMT when 40% of recipient cells was detected. Shortly after he relapsed and died. Among patients who had a related donor there was only one case of transient MC. This study confirmed previous findings that although MC does not necessarily signify imminent relapse it can point out the patients who are at higher risk and help in the determination of the appropriate therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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