Naslov
The role of D6S265, D6S273, and MIB polymorphisms in development of IDDM

Autori
Štingl, Katarina ; Grubić, Zorana ; Žunec, Renata ; Čečuk-Jeličić, Esma ; Radica, Ana ; Dumić, Miroslav ; Brkljačić-Kerhin, Vesna

Izvornik
Genes and Immunity (1466-4879) 4 (2003), 1;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
D6S265; D6S273; MIB; IDDM

Sažetak
Recent investigations have indicated the existence of non-HLA loci associated with IDDM in addition to HLA class II genes. One hundred patients selected on the basis of their HLA class II alleles, where classified in 4 groups (DR3/DR4, DR3, DR4, non DR3/DR4). PCR-SSP method was performed for HLA class II typing, while 3 microsatellites (D6S265, D6S273, and MIB) were analysed using automated ALF express sequencer. The frequency of D6S273 126bp (p=0.02565), 138bp (p=0.01967) and 140bp (p=0.00035) were significantly higher among patients, while the frequency of D6S273 130bp was significantly lower. Analysis of the MIB alleles revealed an increased frequency of alleles 348bp (p=0.01302), 350bp (p=0.00677) and 352bp (p=0.02289) among IDDM, while allele 334bp (p=0.00105) was decreased. There was no difference between patients and controls at D6S265 locus. Comparison of 3 IDDM groups positive for DRB1*0301/*04 with non DRB1*03/*04 patients was also performed, and allele 132bp was present with a significantly higher frequency (p=0.00283) among DRB1*0301/*04 negative patients. This suggests that all other D6S273 and MIB alleles showed association with IDDM because of linkage disequilibrium with DRB1 alleles. When non DRB1*03/*04 group was compared with matched controls, difference in frequency of 132bp allele was also significant (p=0.03986). Our results lead to conclusion that allele 273bp has an independent role in development of IDDM.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA