Naslov
HLA class I and class II polymorphism in Croatian patients with psoriatic arthritis

Autori
Grubic, Zorana ; Perić, Porin ; Čečuk-Jeličić, Esma ; Brkljačić-Kerhin, Vesna ; Ćurković, Božidar ; Kaštelan, Andrija

Izvornik
Annals of the Rheumatic Diseases (0003-4967) 61 (2002), 1;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
psoriatic arthritis; HLA class I and class II

Sažetak
Psoriatic arthritis (PsA) is an inflammatory arthritis associated with psoriasis which is caused by a combination of genetic and environmental factors. The identification of genetic markers for PsA would be of great benefit for diagnosis. The aim of this study was to investigate contribution of HLA-A, -B, -Cw and -DRB1 alleles in the susceptibility to PsA. Fifty-five unrelated patients with PsA (30 women and 25 men, mean age 46 years) were analysed. DNA was isolated by standard salting out protocol, while HLA typing was performed by Polymerase Chain Reaction -Sequence Specific Primers (PCR-SSP) method. The results were compared with a sample of 141 healthy unrelated individuals using Chi-square test with Yates correction and the Fischer's exact test. The relative risk for estimating the strenght of association of the disese with a given allele was calculated by means of Woolf's method. Our results showed that none of 15 distinct HLA-A alleles was increased or decreased in the PsA group, while among 19 different alleles at HLA-B locus 3 alleles showed statistically significant differences between two tested groups. Allele B*13 (7.3% vs 2.6%, p=0.023), B*39 (14.5% vs 2.4%, p=0.0001) and B*27 (15.5% vs 5.0%, p=0.0003) were increased. At HLA-Cw locus, twenty-two patients were Cw*07 (40.4% vs 18.1%, p=0.002) and thirteen patients were positive for Cw*0602 allele (23.1% vs 9.7%, p=0.02). Among HLA-DRB1 alleles, DRB1*0701 showed significant increase in patients with PsA (15.7% vs 7.8%, p=0.019). Results indicated that the higest relative risk (RR) was observed for B*39 (RR=6.19) followed by B*27 (RR=4.25), B*13 (RR=3.40), Cw*07 (RR=3.07), Cw*0602 (RR=2.93) and DRB1*0701 (RR=2.68). The data obtained in this study are consistent with the polygenic inheritance of PsA. Allele B*39 appears to be the strongest genetic susceptibility factor for disease. Further study with larger number of PsA patients is necessary to confirm these findings and to elucidate a possible role of non- HLA loci in the region between HLA B and Cw loci in the susceptibility to PsA.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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