Naslov
Studies of anti-gliadin (AGA) and anti-endomysium (EMA) antibodies in children of short stature

Autori
Tešija, Andrea ; Topić, Elizabeta ; Žižić, Vesna ; Jurčić, Zvonko ; Stipančić, Gordana ; Žaja-Franulović, Orjena ; La Grasta, Lavinia

Izvornik
Clinical chemistry and laboratory medicine. Special supplement (1437-8523) 41 (2003), Special Supplement;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
AGA; EMA; children; short stature

Sažetak
Objective: To investigate the prevalence of celiac disease (CD) in children with short stature using antigliadin (AGA) and antiendomysium (EMA) tests as a screening tools. Patients and methods: A total of 81 children with short stature and no evident gastrointestinal or endocrine disorders were included in the study. IgG and IgA AGA in sera samples were detected by ELISA tests (Euroimmun, Germany and Eurospital, Italy), and IgA EMA by indirect immunofluorescence test using monkey esophagus. Total serum IgA was determined in order to not miss the cases with associated IgA deficiency. A small intestinal biopsy along with HLA typing were used for confirmation of CD in patients with positive serologic tests. Results: 12 out of 81 children (14, 8%) were positive for any of the serologic tests, but 2 of them refused to undergo small intestinal biopsy and were excluded from the study. EMA were positive in 8/10 samples (1 with EMA only, 2 together with AGA-IgG and 5 together with AGA-IgG and AGA-IgA). Two samples negative for EMA were also negative for AGA-IgA while positive for AGA-IgG and was find out to have a selective IgA deficiency. Small intestinal biopsy confirmed CD in all 10 children and 9 of them had a celiac type HLA markers: DR3-DQ2 (6), DR5/7-DQ2 (2) and DR4-DQ8 (1). Conclusion: The frequency of celiac disease in our group (12, 3%) was correspond to other studies. Our results supports the importance of serologic testing for detecting patients that need an intestinal biopsy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija

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