Naslov
Coordination of metal ions in the 2-Mo and 2-Ni type of insulin

Autori
Prugovečki, Biserka ; Matković-Čalogović, Dubravka ; Dodson, Eleanor ; Dodson, Guy

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
21st European crystallographic meeting : Abstracts : u: Acta Crystallographica. section A 59 (2003) (S) dok je ISSN 0108-7673 / Liles, Dave ; Dillen, Jan - Durban : ConferenceCall, 2003, 126-126

Skup
European crystallographic meeting (21 ; 2003)

Mjesto i datum
Durban, Južnoafrička Republika, 24.08.2003. - 29.08.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
insulin; metal coordination; metal replacement

Sažetak
Insulin is a metalloprotein and in the rhombohedral crystalline form named 2-Zn insulin it is organised as a hexamer with the zinc ion octahedrally coordinated by three His imidazole ligands and three water molecules. It has been known for many years that different ions can substitute the zinc ion [1], however only a few of such complexes have been structurally characterized [2]. In the present study the zinc ion in insulin was substituted with Mo6+ and Ni2+ ions in order to investigate the difference in their metal coordination. Crystals of these insulin derivatives were obtained from zinc-free insulin under conditions analoguous to those for 2-Zn insulin but with addition of the above mentioned metal salts. The X-ray diffraction data have been collected at the synchrotron Elettra in Trieste for the 2-Ni insulin to 1.3 Å ; ; ; and at ESRF in Grenoble for the 2-Mo insulin to 1.1 Å ; ; ; resolution. In the 2-Mo insulin, one of Mo6+ ions is octahedrally coordinated by three His imidazole ligands and three water molecules, while the other is disordered with both octahedral and tetrahedral coordination present. In the 2-Ni insulin both Ni2+ ions are octahedrally coordinated by three His imidazole ligands and three water molecules. [1] T. Blundell, G. Dodson, D. Hodgkin & D. Mercola, Adv. Protein Chem. 26 (1972) 279-402. [2] C. P. Hill, Z. Dauter, E. J. Dodson, G. Dodson & M. F. Dunn, Biochemistry 30 (1991) 917-924.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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