Naslov
Mutations in SCW and PIR genes influence cell viability: synergists and antagonists in the cell wall of S. cerevisiae

Autori
Teparić, Renata ; Borić, Vladko ; Mojzeš, Andrijana ; Slivac, Igor ; Mrša, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
II International Conference on Molecular Mechanisms of Fungal Cell Wall Biogenesis / Duran, A. - Salamanca : University of Salamanca, 2003

Skup
II International Conference on Molecular Mechanisms of Fungal Cell Wall Biogenesis

Mjesto i datum
Salamanca, Španjolska, 27.08.2003. - 01.09.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Yeast cell wall; Cell wall proteins; SCW4; SCW10; SCW11; BGL2; PIR-proteins
(yeast cell wall; cell wall proteins; SCW4; SCW10; SCW11; BGL2; PIR-proteins)

Sažetak
Saccharomyces cerevisiae cell wall mannoproteins can either be noncovalently (Scw proteins - soluble cell wall proteins), or covalently (Ccw proteins - covalently linked cell wall proteins) bound to glucan. Ccws are usually extracted by glucanases but a group of them (so called Pir - proteins with internal repeats) can also be released from glucan by a treatment with NaOH. Physiological role of most cell wall proteins is still unknown. In order to assess possible role of cell wall proteins, mutants were constructed by disruption of SCW4, SCW10, SCW11, and SCW8/BGL2, as well as all four known PIR genes (CCW5, CCW6/PIR1, CCW7/PIR2/HSP150, and CCW8/PIR3). Viability of mutants in exponential and stationary phase of growth was monitored by methylene blue staining. It was shown that exponentially growing scw4scw10 and scw4scw10bgl2 showed significantly increased fraction of dead cells in the culture. Interestingly, additional scw11 mutation suppressed the observed phenotype indicating an antagonistic behavior of Scw11p to Scw4p, Scw10p and Bgl2p. In agreement with this, it was also found that scw4scw10 secretes less proteins into the growth medium, which was also reversed by an additional scw11 mutation. Similar relation between the strains was found if cells were grown to stationary phase but the difference to wild type was more pronounced suggesting that possible role(s) of these proteins may be more important for stationary cells. Successive mutations of PIR genes also led to increased mortality of cells and similar to other phenotypes observed previously, it also showed cumulative effect. Unlike the scw mutations, in this case young cells seem to be more affected. Microscopic investigation showed an increased fraction of cells with more than one bud and in most cases daughter cells still attached to their mothers stained with methylene blue.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA