Naslov
The PMTs: an evolutionarily conserved family of protein O-mannosyltransferases

Autori
Girrbach, Verena ; Lommel, Mathias ; Ecker, Margit ; Mrša, Vladimir ; Tanner, Widmar ; Strahl, Sabine

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
II International Conference on Molecular Mechanisms of Fungal Cell Wall Biogenesis / Duran, A. - Salamanca : University of Salamanca, 2003

Skup
II International Conference on Molecular Mechanisms of Fungal Cell Wall Biogenesis

Mjesto i datum
Salamanca, Španjolska, 27.08.-01.09

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Protein glycosylation; O-glycosylation; Yeast mannoproteins
(protein glycosylation; O-glycosylation; yeast mannoproteins)

Sažetak
Protein O-mannosylation is an essential protein modification in yeast. Furthermore, it is indispensable for cell morphology and cell wall integrity. Yet, how O-mannosylation affects cell wall architecture is still obscure. In yeasts protein O-mannosylation is initiated at the endoplasmic reticulum by an evolutionarily conserved family of protein O-mannosyltransferases, the PMTs. Phylogenetic analyses revealed that PMTs can be divided into the PMT1, PMT2 and PMT4 subfamily. Here we discuss distinct features of PMT1/PMT2 and PMT4 subfamily members, such as molecular organization and substrate specificities. Further, characterizing pmt mutants we found that their phenotype closely resembles mutants of the cell wal integrity signaling (PKC1-) pathway which monitors cell wal stability. Analysis of the PKC1-pathway revealed that O-mannosylation is essential for induction of the this signal transduction cascade upon external stresses. Biochemical analysis of the highly O-mannosylated upstream receptors of the cell wall integrity pathway showed the abated O-mannosylation affects their maturation, stability and function.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA