Naslov
Myxovirescin Biosynthesis: An intriguing megasynthetase consisting of polyketide synthases, nonribosomal peptide synthetase, 3-hydroxy-3-methylglutaryl-CoA synthases and trans-acting acyltranferases

Autori
Simunovic, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Proceedings of the 32nd International Conference on the Biology of the Myxobacteria / - , 2005, 42-42

Skup
32nd International Conference on the Biology of the Myxobacteria

Mjesto i datum
Harrison Hot Springs (BC), Kanada, 10.07.2005. - 13.07.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Myxovirescin Biosynthesis

Sažetak
Myxobacteria produce a variety of secondary metabolites displaying important biological activities. Recent sequencing of the Myxococcus xanthus DK1622 genome revealed its high potential for the production of secondary metabolites and led to the identification of the myxovirescin biosynthetic gene cluster. In silico analysis of myxovirescin megasynthetase resulted in the proposal that a number of discrete enzymes work together with the multimodular PKS to build myxovirescin scaffold, unique for the presence of two different β-alkyl groups. To test the myxovirescin biosynthetic model, fourteen in-frame deletion mutants in the myxovirescin biosynthetic gene cluster were created, and their effects on the production of myxovirescin antibiotics evaluated by HPLC-MS analysis of the resulting mutant extracts. Novel myxovirescin analogues arising from certain mutant backgrounds were structurallyelucidated to identify the specific positions of these modifications. In silico analysis of an additional 11 kb region encoded upstream from the myxovirescin gene clusters were proposed to be involved in the regulation of its production. Genetic disruption of a gene encoding for a serine/threonine kinase, and two additional genes encoding for proteins of unknownfunctions, were shown to positively regulate myxovirescin production.

POVEZANOST RADA