Pregled bibliografske jedinice broj: 1282393
Observing membrane and cell adhesion via Reflection Interference Contrast Microscopy
Observing membrane and cell adhesion via Reflection Interference Contrast Microscopy // The Immune Synapse: Methods and Protocols (Second edition) / Baldari, C. T. ; Dustin, M. L. (ur.).
USA: Springer US, 2023. str. 123-136 doi:10.1007/978-1-0716-3135-5
CROSBI ID: 1282393 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Observing membrane and cell adhesion via Reflection Interference Contrast Microscopy
Autori
Ahmed, Abdelrahman ; Ana-Sunčana, Smith ; Kheya, Sengupta
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni
Knjiga
The Immune Synapse: Methods and Protocols (Second edition)
Urednik/ci
Baldari, C. T. ; Dustin, M. L.
Izdavač
Springer US
Grad
USA
Godina
2023
Raspon stranica
123-136
ISBN
978-1-0716-3134-8
ISSN
1064-3745
Ključne riječi
Interferometry ; Adhesion ; Fluctuations ; Membranes ; Antiflex
Sažetak
Reflection interference contrast microscopy (RICM) is an optical microscopy technique ideally suited for imaging adhesion. While RICM (and the closely related interference reflection microscopy (IRM)) has been extensively used qualitatively or semiquantitatively to image cells, including immune cells, it can also be used quantitatively to measure membrane to surface distance, especially for model membranes. Here, we present a protocol for RICM and IRM imaging and the details of semiquantitative and quantitative analysis.
Izvorni jezik
Engleski
Znanstvena područja
Fizika
Citiraj ovu publikaciju:
Časopis indeksira:
- Scopus