Naslov
Amphiphilic cationic tripyridiniumporphyrins and their Zn(II) complexes: the influence of the irradiation wavelength and the length of the alkyl chain

Autori
Mušković, Martina ; Lončarić, Martin ; Ratkaj, Ivana ; Malatesti, Nela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
23rd Annual Conference of the Italian Society of Photobiology

Mjesto i datum
Lecce, Italija, 28.06.2023. - 30.06.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Photodynamic therapy ; Amphiphilic porphyrins ; Singlet oxygen ; Melanoma cells

Sažetak
Amphiphilic porphyrins are increasingly proving to be effective photosensitizers (PSs) and promising for the use in photodynamic therapy (PDT) [1]. In our previous research, cationic porphyrins conjugated with a long alkyl chain (18C) showed much higher cytotoxicity on different cell lines compared to a hydrophilic analogue with an acetamido group [2]. Chelation with zinc(II) ion is expected to increase the lifetime of the triplet excited state (3PS*) and improve singlet oxygen production [3]. Since chelation reduces the number of Q bands from four to two with negligible absorption above 610 nm, activation of Zn(II) porphyrins with red light common to PDT is unlikely to be effective, while orange light is much better suited to their optical properties, which still penetrates tissue relatively well. We will present the preparation of free-bases and Zn(II) complexes of N-methylated tripyridinium-3-ylporphyrins conjugated with alkyl chains of different lengths (1C, 8C-18C) and a study of the influence of their lipophilicity on PDT activity against melanoma cell lines. This study includes the determination of photophysical and photochemical properties in different solvents using various spectroscopic methods (UV-VIS and fluorescence spectroscopy, laser flash photolysis (LFP) and time-resolved fluorescence spectroscopy (TRF)) as well as in vitro biological analyses on two different melanoma cell lines (A375 and MeWo) and human dermal fibroblasts (HDF), where cellular uptake of each PS, its intracellular localisation and cytotoxicity were investigated. In addition, the MTT assay was used to compare the application of two different wavelengths for photoactivation of the PSs, red (645 nm ; 2.0 mW / cm2 ) and orange light (607 nm ; 2.0 mW / cm2).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA