Pregled bibliografske jedinice broj: 1280614
A capillary electrophoresis and off-line capillary electrophoresis/electrospray ionization-quadrupole time of flight-tandem mass spectrometry approach for ganglioside analysis
A capillary electrophoresis and off-line capillary electrophoresis/electrospray ionization-quadrupole time of flight-tandem mass spectrometry approach for ganglioside analysis // Electrophoresis, 23 (2002), 17; 2894-2903 doi:10.1002/1522-2683(200209)23:173.0.CO ; 2-Q (međunarodna recenzija, članak, znanstveni)
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Naslov
A capillary electrophoresis and off-line capillary
electrophoresis/electrospray ionization-quadrupole
time of flight-tandem mass spectrometry approach
for ganglioside analysis
Autori
Zamfir, Alina ; Vukelić, Željka ; Peter-Katalinić, Jasna
Izvornik
Electrophoresis (0173-0835) 23
(2002), 17;
2894-2903
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
capillary electrophoresis ; electrospray ionization ; gangliosides ; tandem mass spectrometry
Sažetak
A systematic study for the optimization and implementation of high-performance capillary electrophoresis (HPCE) in conjunction with negative ion electrospray ionization-quadrupole time of flight-tandem mass spectrometry (ESI-QTOF- MS/MS) for the analysis of complex glycolipids is described. The performance of the capillary electrophoresis (CE) and off-line CE/ESI-QTOF-MS approach has been explored for screening a complex ganglioside mixture from bovine brain. All instrumental and solution parameters demonstrated to require special adjustment and to have the most substantial effect on the CE separation, abundance of product ions produced in a low-energy collision-induced dissociation (CID) process and their detection by MS/MS, when attempting to identify and sequence single ganglioside molecular species from CE eluted fractions. Upon optimization of the experimental parameters, an efficient methodology emerged providing the general basic requirements for combined CE/ESI-MS analysis of this type of complex glycoconjugate.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Poveznice na cjeloviti tekst rada:
doi analyticalsciencejournals.onlinelibrary.wiley.com www.webofscience.comCitiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
