Naslov
In vitro quality properties of platelets pooled and stored in three different pooling system

Autori
Liker, Milica ; Strauss Patko, Maja ; Tomičić, Maja ; Gulan Harcet, Jadranka ; Gojčeta, Koraljka ; Jukić, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Vox Sanguinis, The International Journal of Transfusion Medicine, vol 111 (Suppl.1) / - , 2016, 150-150

Skup
34th International Congress of ISBT

Mjesto i datum
Dubai, Ujedinjeni Arapski Emirati, 03.09.2016. - 08.06.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
trombociti, hranjiva otopina
(platelets, additive solution)

Sažetak
AIMS Comparation in vitro properties of platelets stored in plasma and two different additive solutions (Intersol and SSP+) for 5 days. The storage of platelets concentrate in plasma is well-establish practice and provide a benchmark against which the performance of platelets stored in additive solutions can be compared. METHODS Donor differences were avoided by applying pool- and-split design. Twelve buffy coats were pooled and divided into three parts. Each part was pooled with 240 mL of plasma or 250 mL of PASs (Intersol, Fresenius Kabi or SSP+, Macopharma) using pooling system of Haemonetics. On days 1 and 5 of storage samples were tested for platelets concentration, mean platelet volume (MPV), pO2, pCO2, glucose, lactate, bicarbonate, pH, CD62P, ADP induced aggregation. RESULTS The results were summarized in the Table. All platelets concentrates met national and Council of Europe guidelines for platelet products. No significant differences were observed for pH, MPV, pO2 between PCs stored in plasma and PCs stored in platelet additive solutions. PCs stored in plasma showed higher platelets count, glucose and bicarbonate level and ADP induced aggregation. PCs stored in SSP+ showed better quality compared to PCs stored in Intersol as indicated by better platelets count, lower glucose consumption and lower platelets activation. CONCLUSION In vitro quality of PCs stored in both platelet additive solution is well maintained. However, storage of PCs in Intersol results in increased platelet activation and platelet metabolism compared with PCs stored in SSP+.

Izvorni jezik
Engleski

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