Naslov
CORRELATIONS BETWEEN PHYTOCHEMICAL AND BIOLOGICAL ACTIVITY PARAMETERS IN BROCCOLI AFTER IN VITRO DIGESTION: INFLUENCE OF PLANT DEVELOPMENTAL STAGE

Autori
Šola, Ivana ; Vujčić Bok, Valerija ; Rusak, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ISPEC 8th INTERNATIONAL CONFERENCE ON AGRICULTURE, ANIMAL SCIENCES and RURAL DEVELOPMENT - Book of Abstracts / - , 2021, 3-3

Skup
ISPEC 8th INTERNATIONAL CONFERENCE ON AGRICULTURE, ANIMAL SCIENCES and RURAL DEVELOPMENT

Mjesto i datum
Istanbul, Turska, 23.12.2021. - 25.12.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Brassica oleracea L. convar. botrytis, PCA, polyphenols, glucosinolates, sugars, in vitro digestion, antioxidant activity, antidiabetic activity, obesity

Sažetak
The principal component analysis (PCA) was used to gain insight into the relationship between phytochemicals (total phenols - TP, total phenolic acid - TPA, total flavonoids -TF, total anthocyanidins - TA ; total hydroxycinnamic acids - THA, and total flavanols - TFL, glucosinolates – Gls, sugar – TS) and antioxidant, antidiabetic and anti-obesity activity in three developmental stages of broccoli after in vitro digestion. Different developmental stages of broccoli (1st stage: seedlings, 2nd stage: plants with 2 real leaves, and 3rd stage: plants with 4-6 real leaves) were collected in July and August 2021, and extracts were prepared in hot 70% ethanol. In PCA the first and the second principal components described 64.62% and 35.38% of the variance after in vitro digestion, respectively. Together, the first two PCs represent 100% of the total variability. After digestion, 1st developmental stage of broccoli had strong loadings with most of the tested chemicals (TA, Gls, TS), DPPH and inhibition of alpha-amylase. Broccoli 2nd developmental stage had strong loadings with TPA and inhibition of lipase, while 3rd developmental stage had strong loadings with TF and inhibition of alpha-glucosidase. Pearson’s correlation coefficient between phytochemicals, antioxidant (ABTS, DPPH and FRAP), antidiabetic (inhibition of alpha-amylase and alpha-glucosidase) and anti-obesity activity (lipase inhibition) was also performed. After digestion, TP correlated very strongly (0.999) with THA, TPA correlated very strongly with antioxidant (FRAP: 0.998) and anti-obesity activity (inhibition of lipase: 1.000), TA correlated very strongly alpha1.000) with antidiabetic activity (inhibition of alpha-amylase), Gls correlated very strongly with antioxidant activity (DPPH: 0.999), and DPPH correlated very strongly (0.997) with alpha-amylase. According to the results after in vitro digestion, 1st developmental stage of broccoli was the best source of TA, Gls and TS, that are closely related with antioxidant activity (DPPH) and key enzyme linked to type-2 diabetes (alpha-amylase). 2nd developmental stage of broccoli was the best source of TPA, that are closely related with inhibition of key enzyme linked to obesity (lipase) ; and 3rd developmental stage was the best source of TF, that are closely related with inhibition of key enzyme linked to type-2 diabetes (alpha-glucosidase).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA