Naslov
Influence of temperature stress on the concentration of vitamin C and phenolic compounds in broccoli (Brassica oleracea botrytis var. cymosa)

Autori
Kokić, Emilie: Zekirovski, Jana ; Šola, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SiSB Book of Abstracts 2022 / - Zagreb : University of Zagreb, Faculty of Science, 2022, 82-83

Skup
Simpozij studenata bioloških usmjerenja 2022

Mjesto i datum
Zagreb, Hrvatska, 21.05.2022. - 22.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
flavonoids ; phenolic acids ; temperature stress ; vegetables ; vitamin C

Sažetak
Broccoli (Brassica oleracea botrytis var. cymosa) was grown to a stage with two adult leaves, after which it was subjected to temperature stress until the stage of development with 6-8 adult leaves. Ice water treatment was performed by applying ice to the substrate in pots in which broccoli was grown and hot water treatment by watering the substrate with water at a temperature of 80°C. A control group of plants was watered with room temperature water. After collecting the aboveground part of the plants, it was immediately frozen in a stream of liquid nitrogen and then lyophilized. The extracts were prepared in 70% ethanol, after which they were hydrolysed using 1.2 M hydrochloric acid. Separation, identification and quantification of vitamin C, ferulic and synapic acid and flavonols quercetin and kaempferol were performed using high performance liquid chromatography (HPLC). Stress caused by high temperature water significantly increased the concentration of vitamin C and ferulic acid and decreased the concentration of kaempferol and synapic acid in young broccoli plants. Stress caused by ice water did not increase the concentration of any of the analysed bioactive compounds, but therefore significantly reduced the concentration of kaempferol and synapic acid. The concentration of flavonol quercetin in young broccoli did not change significantly after stress caused by hot and ice water.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

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