Naslov
Synthesis of galactooligosaccharides in batch and enzyme membrane reactor

Autori
Mateša, Lorena ; Leaković, Emerik ; Bigor, Ivana ; Sudar, Martina ; Vrsalović Presečki, Ana ; Findrik Blažević, Zvjezdana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28 th Croatian Meeting of Chemists and Chemical Engineers, Book of Abstracts / Rogošić, Marko - Zagreb, 2023, 161-161

Skup
28 th Croatian Meeting of Chemists and Chemical Engineers

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
galactooligosaccharides, batch reactor, enzyme membrane reactor

Sažetak
Galactooligosaccharides (GOSs) are nondigestible oligosaccharides composed of a variable number of galactose units (from two to nine) linked by different glycosidic bonds to the terminal glucose. Many health benefits have been claimed for GOSs that are referred both to gut health and immune modulation. [1] The chemical synthesis of GOSs is laboured process, and uneconomical on a commercial scale, which is why the synthesis on a commercial scale is mostly performed using biocatalysis. [2] Traditionally, GOSs are produced in stirred tank reactors, but this setup includes inactivation and subsequent separation of the biocatalyst to achieve enzyme-free GOS products. An alternative to batch processes is the application of enzyme membrane reactor, where the membrane retains the enzyme while lower weight saccharides pass through it. As a result, simultaneous GOS synthesis and biocatalyst separation can be achieved. [3] In this work, transgalactosylation reaction catalysed by βgalactosidase from Aspergillus oryzae (Fig 1.) in the batch and enzyme membrane reactor was studied. As substrate, lactose solution in water and 0.9% fat milk were used. Initial concentration of lactose was chosen to be similar to the concentration of lactose in milk. Operational stability of β-galactosidase was measured during the reaction in both reactor modes. Enzyme membrane reactor is known as a convenient way of a continuous production process with enzyme reuse. [3] Once the optimal operational parameters are established, it might serve as a promising alternative to conventional batch-mode reactor.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA