Naslov
Ultrasound assisted-enzyme based hydrolytic microextration as a simple and green method for determination of trace levels of manganese in foodstuffs by total reflection X-ray spectrometry

Autori
Margui, Eva ; Bahadir, Zekeriya ; Jablan, Jasna ; Queralt, Ignasi

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EXRS-2022_Book of Abstract / - , 2022, 109-109

Skup
2022 European Conference on X-Ray Spectrometry

Mjesto i datum
Brugge, Belgija, 26.06.2022. - 01.07.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
enzyme based hydrolytic microextration ; TXRF ; manganese

Sažetak
Manganese is an essential dietary nutrient that plays an important role in many biochemical reactions in human body and it is mainly obtained from foodstuff. Therefore, its determination in different types of food samples is of special interest for nutritional studies. Usually, the determination of manganese in food is performed by acid digestion followed by detection using a sensitive spectroscopic analytical technique. In the last years, efforts have been made to incorporate new screening or quantitative low-cost analytical tools which are more in line with the green and sustainable analytical chemistry principles. Enzymatic hydrolysis extraction, based on the transfer of analyte from the solid food matrix to an aqueous phase, is a good sample treatment procedure to be explored. In the present contribution, a simple and green ultrasound assisted-enzyme based hydrolytic microextraction (UA-EH-ME) method has been developed and used in combination with total reflection X-ray spectrometry (TXRF) for the quantification of trace levels of Mn in different types of food samples. The main benefits of the proposed approach include the use of a small amount of innocuous reagents (mg level) and the speed of the whole method (30 min). Firstly, a carefully evaluation of the best extraction and measurement TXRF conditions was carried out, including also a -EDXRF study of the shape and element distribution of the deposited residue on the reflector. Different quantification approaches were also tested including internal standardization and empirical calibration using a set of food reference materials. The analytical capabilities of the UA-EH-ME-TXRF method were compared with those associated with other sample treatments, such as suspension and digestion, commonly used in solid sample analysis by TXRF. Finally, the developed method was applied to the analysis of several real food samples and the obtained results were compared with those determined by inductively coupled plasma optical emission spectroscopy (ICP-OES) after microwave digestion of the sample. No significant statistical differences were found between concentrations determined using both methodologies.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA