Naslov
Potential of total-reflection X-ray spectrometry for multielement analysis of biological samples using dilution or suspension sample preparation techniques

Autori
Margui, Eva ; Jablan, Jasna ; Queralt, Ignasi ; Bilo, Fabjola ; Borgese, Laura

Izvornik
X-Ray Spectrometry (0049-8246) 51 (2021); 1-11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
biological samples ; TXRF ; multi elemental analysis ; sample preparations

Sažetak
In most clinical and nutritional studies, it is of significance to know information about the multielemental composition of biological samples. Conventional analysis of biological samples relies upon sample digestion followed by atomic spectrometry detection. This approach is essential for the quantification of ultratrace elements in biological samples. While in other applications it could be of interest to have simpler analytical methods withmultielemental capability but involvingaminimumsample treatment, reduce the amount of sample and a more cost-effective analysis. In the present contribution, the possibilities and drawbacks of simple sample treatments (i.e., dilution and suspension) in combinationwithtotal reflectionX- ray fluorescence spectrometry (TXRF) for the analysis of different types of biological samples have been critically evaluated. For that, a set of reference materials or well-characterized biological human fluids (blood, serum, plasma and seminal plasma) and animal/ vegetal tissues have been used to estimate the analytical capabilities in terms of limits of detection, trueness and precision of the proposed TXRF methods. The results are based on the authors' experience in analysing biological samples using TXRF, and it is expected that they can be useful for new TXRF users in this field and they can provide a good basis for further application of this technique in clinical studies and other applications dealing with the analysis of biological samples in the future.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA