Naslov
Neuroprotective effect of BDNF in an in vitro model of Alzheimer’s disease

Autori
Vuić, Barbara ; Nikolac Perković, Matea ; Nedić Erjavec, Gordana ; Tudor, Lucija ; Konjevod, Marcela ; Pivac, Nela ; Švob Štrac, Dubravka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Virtualni 8. Hrvatski kongres neuroznanosti : knjiga sažetaka / - , 2021, 55-55

Skup
Virtualni 8. Hrvatski kongres neuroznanosti

Mjesto i datum
Zagreb, Hrvatska, 24.09.2021. - 25.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Aβ oligomers ; Alzheimer´s disease ; BDNF ; neuroprotection ; primary mouse neurons

Sažetak
Alzheimer´s disease (AD) is a multifactorial neurodegenerative disorder still without effective and stable therapeutic strategies. It is characterized by the progressive cognitive decline and neuronal death, especially in the hippocampus and neocortex, which is probably due to the accumulation of beta amyloid (Aβ) plaques and neurofibrillary tangles. The etiology of AD is still not clear ; however, there are many known genetic and environmental risk factors. Brain- derived neurotrophic factor (BDNF) is the most widely distributed neurotrophic factor in the adult brain, whose levels are reduced in AD. BDNF plays an important role in synaptic plasticity, synaptogenesis, neuronal survival and growth, particullary in the hippocampus, the brain region essential for learning and memory. This study has investigated potential neuroprotective effect of BDNF in primary neuronal cultures derived from the C57BL/6 mice. Primary mouse neurons were treated with various concentrations of toxic Aβ oligomer preparations, as well as with different BDNF concentrations. The colorimetric MTT assay, Promega cell-based and biochemical assays and Muse cell analyzer were used to investigate Aβ-induced cytotoxicity, as well as neuroprotective effects of BDNF. The results demonstrated that Aβ oligomers caused necrosis and apoptosis of neuronal cells, rather than oxidative stress, presumably acting via membrane integrity disruption, as well as caspase and bcl-2 activation. The observed neuroprotective effect of BDNF alone, but also in the combination with neurosteroids dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) is, at least partly, achieved by blocking the caspase activation and suggests beneficial antiapoptotic actions of BDNF on the survival of neurons damaged in AD.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA