Pregled bibliografske jedinice broj: 1274764
The poor ends-out recombination fidelity in budding yeast may be rescued by altering the orientation of the insertion within the targeting module
The poor ends-out recombination fidelity in budding yeast may be rescued by altering the orientation of the insertion within the targeting module // Zbornik sažetaka 14. hrvatskog biološkog kongresa s međunarodnim sudjelovanjem = Book of abstracts of the 14th Croatian biological congress with International Participation / Caput Mihalić, Katarina ; Mičetić Stanković, Vlatka ; Urlić, Inga ; Mešić, Armin ; Kružić, Petar (ur.).
Zagreb: Hrvatsko biološko društvo, 2022. str. 12-13 (predavanje, domaća recenzija, sažetak, znanstveni)
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Naslov
The poor ends-out recombination fidelity in budding yeast may be rescued by altering the orientation of the insertion within the targeting module
Autori
Mitrikeski, Petar Tomev
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Zbornik sažetaka 14. hrvatskog biološkog kongresa s međunarodnim sudjelovanjem = Book of abstracts of the 14th Croatian biological congress with International Participation
/ Caput Mihalić, Katarina ; Mičetić Stanković, Vlatka ; Urlić, Inga ; Mešić, Armin ; Kružić, Petar - Zagreb : Hrvatsko biološko društvo, 2022, 12-13
Skup
14. Hrvatski biološki kongres s međunarodnim sudjelovanjem = 14th Croatian biological congress with International Participation
Mjesto i datum
Zagreb, Hrvatska, 12.10.2022. - 16.10.2022
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
yeast Saccharomyces cerevisiae ; ends-out recombination ; one-step gene disruption ; gene targeting ; insertion orientation ; transcription
Sažetak
Gene-targeting is the most important molecular tool for genome manipulations. However, the efforts for reliable genetically modified genomes are undermined by many factors influencing the ratio between the allelic and the ectopic recombination that controls the targeting fidelity. The known fact that this ratio is usually low in non-Saccharomyces yeasts and, most importantly, metazoans renders the understanding of the targeting mechanisms one of the key goals of contemporary genetics. Therefore, in this study I compared earlier side results of molecular construction of yeast strains with the contemporary knowledge focusing on the orientation of the insertion within the cassette for two reasons: (i) researchers usually choose the insertion orientation at random, and (ii) the possible influence of the parameter has never before been analyzed systematically. Thus, here I show that the cassettes bearing divergently oriented insertions significantly remedied mistargeting caused by their convergent counterparts in two loci (URA3 and LEU2) and one allele (ura3-52) in wt yeast cells (Mitrikeski, 2022, Curr. Genet., in press). It is tempting to suggest that the effect might be transcription-related. If so, this could potentially open a new area of genetics.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Fakultet filozofije i religijskih znanosti
Profili:
Petar Mitrikeski
(autor)
