Pregled bibliografske jedinice broj: 1274337
BEGINNERS GUIDE TO SAMPLE PREPARATION TECHNIQUES FOR TRANSMISSION ELECTRON MICROSCOPY
BEGINNERS GUIDE TO SAMPLE PREPARATION TECHNIQUES FOR TRANSMISSION ELECTRON MICROSCOPY // Periodicum biologorum (2023) (pregledni, prihvaćen)
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Naslov
BEGINNERS GUIDE TO SAMPLE PREPARATION TECHNIQUES FOR
TRANSMISSION ELECTRON MICROSCOPY
Autori
Dumančić, Ena ; Vojta, Lea ; Fulgosi, Hrvoje
Vrsta, podvrsta
Radovi u časopisima,
pregledni
Izvornik
Periodicum biologorum (2023)
Status rada
Prihvaćen
Ključne riječi
TEM ; sample preparation ; fixation ; resin embedding ; contrasting ; ultramicrotome
Sažetak
The revolution in microscopy came in 1930 with the invention of electron microscope. Since then, we can study specimens on ultrastructural and even atomic level. Besides transmission electron microscopy (TEM), there are other types of electron microscopes that are not discussed in this review. Here, we have described basic procedures for TEM sample preparation, which include tissue sample preparation, chemical fixation of tissue with fixatives, cryo-fixation performed by quick freezing, dehydration with ethanol, infiltration with transitional solvents, resin embedding and polymerization, processing of polymerized specimens, sectioning of samples with ultramicrotome, positive and negative contrasting of samples, immunolabeling, and imaging. Such collection of methods can be useful for novices in transmission electron microscopy.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija
POVEZANOST RADA
Projekti:
HRZZ-IP-2020-02-8590 - Procjena fotosintetske aktivnosti i fotozaštitnog potencijala TROL-FNR razdjelnice (FUTURE PLANT) (Fulgosi, Hrvoje, HRZZ - 2020-02) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
