Naslov
OPTIMIZING THE REACTION CONDITIONS OF ENZYME – CATALYSED BETULOSIDE HYDROLYSIS

Autori
Leaković, Emerik ; Thier, Isabel ; Siems, Karsten ; Mateša, Lorena ; Sudar, Martina ; Findrik Blažević, Zvjezdana ; Vrsalović Presečki, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28 HSKIKI Book of Abstracts / - , 2023, 158-158

Skup
28th Croatian Meeting of Chemists and Chemical Engineers (28HSKIKI)

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Betuloside, biocatalysis, hydrolysis

Sažetak
Raspberry ketone is a natural phenolic compound found in the red raspberry (Rubus idaeus) and is widely used in the food and cosmetic industries. [1] Precisely because of its various market applications, the production of raspberry flavourings is an important trend. The amount of raspberry ketone in the fruit is extremely low, which makes direct isolation of the compound economically unviable. [2] A promising production method is a biocatalytic process that uses as substrate rhododendrol glycosides, e.g. betuloside, precursors found in inner birch bark. Hydrolysis of rhododendrol glycosides results in the (R)- and (S)-rhododendrol, which can be enzymatically converted to raspberry ketone. [3] In this study, the reaction of betuloside hydrolysis by β-glucosidase from almonds (Figure 1.) in a batch reactor was studied. The reaction conditions including buffer, pH and temperature were examined. The kinetic parameters were estimated under the optimal conditions of the previously defined parameters. To confirm the developed mathematical model and estimated kinetic parameters, the reaction was carried out in a batch reactor.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA