Naslov
Glycans as biomarkers and funcƟonal effectors in diabetes

Autori
Gornik Kljaić, Olga

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
45th FEBS Congress

Mjesto i datum
Online ; Ljubljana, Slovenija, 03.07.2021. - 08.07.2021

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
glycosylation, diabetes

Sažetak
Accumulating evidence is demonstrating that N­ glycosylation participates in various processes involved in the regulation and maintenance of glucose homeostasis and is tightly connected to nutrient sensing through the hexosamine biosynthetic pathway. Distinctive N­ glycosylation profiles are reported for different diabetes subtypes. Proinflammatory­like changes of plasma N­glycome were noticed in cross­sectional studies of type 2 diabetes mellitus (T2DM) and N­ glycans showed the ability in identifying individuals at high risk of disease development. Individuals with a higher risk due to recorded hyperglycemia through acute illness had increased complexity of plasma N­glycome, possibly reflecting the altered flux of glucose through the hexosamine pathway. This was subsequently confirmed in two prospective studies with incident T2DM showing that N­glycome could predict diabetes years before clinical manifestation. N­ glycome also showed great discriminative power in maturity­onset diabetes of the young where triantennary sialylated plasma N­glycan with antennary fucose was successful in extracting individuals with damaging HNF1A mutations. As this phenomenon is also present on alpha­1 acid glycoprotein, targeted analysis of specific glycopeptides could easily be incorporated in diagnostic procedures due to the increasing availability of LC­MS/MS in hospital laboratories. Recently, we also analyzed glycosylation in children with type 1 diabetes mellitus (T1DM). Associations between glycan structures and genetic polymorphisms previously associated with T1DM risk were found. Differences in N­glycomes between children with T1DM and their healthy siblings possess great discriminative potential, which exceeds other predictive models used and is comparable to the most recently established genetic risk score.

Izvorni jezik
Engleski

POVEZANOST RADA