Naslov
Effects of coencapsulated NGF and GM1 in rats with cortical lesions

Autori
Maysinger, Dušica ; Filipović-Grčić, Jelena ; Cuello, A. Claudio

Izvornik
NeuroReport (0959-4965) 4 (1993), 7; 971-974

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
coencapsulation ; neurotrophin delivery ; nerve growth factor ; monosialoganglioside ;

Sažetak
Nerve growth factor (NGF) and monosialoganglioside GM1 were microencapsulated in biodegradable co-polymer poly(L-lactide):co- glycolide and administered locally to rats with unilateral cortical devascularizing lesions. Microcapsules were placed directly onto the lesioned cortical surface and rats were sacrificed 30 days post- operatively. Biochemical and quantitative immunocytochemical analyses revealed effective protection from degenerative changes of nucleus basalis magnocellularis (NBM) cholinergic neurones on the lesioned side as well as a significant increase in choline acetyltransferase activity in the frontoparietal cortex surrounding the lesion. Results of this study strongly suggest that the topical application of microcapsules of a biodegradable polymer containing a mixture of two neuroprotective factors is a viable alternative to the use of osmotic minipumps for delivery of these agents into the CNS. Indeed, this approach might find clinical applications.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA