Naslov
Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites

Autori
Lenić, Ines

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Farmaceutsko-biokemijski fakultet

Mjesto
Zagreb

Datum
29.09

Godina
2015

Stranica
42

Mentor
Nigović, Biljana

Neposredni voditelj
Sikanen, Tiina

Ključne riječi
Mikročip, elektroforeza, elektrokemijska detekcija, analiza lijekova i metabolita
(Microchip, electrophoresis, electrochemical detection, µTAS, screening, substrates, metabolites)

Sažetak
In a period of rapidly growing pharmaceutical industry development of the current analytical techniques is by far too slow to fulfil requests of demanding metabolic analysis of new pharmaceuticals and new chemical entities (environmental and household chemicals). Portable Microchip Capillary Electrophoresis (MCE) platform with amperometric electrochemical detection (EC) was developed in this work, offering a new approach to analytical chemistry. By following Micro Total Analysis System (µTAS) concept, MCE-EC platform combines rapid analysis, low price and practicality with low requirements of reagents. Research was made by using compact platform for MCE-EC purchased from MicruX Techologies. It was developed for chosen drug/metabolite pairs (phenacetin/paracetamol, coumarin/umbelliferone and diclofenac/4'-hydroxydiclofenac). Different voltages, analysis times and system setups were tested. In addition to this, different on-chip sample preparation and purification methods (liquid phase microextraction and electrokinetic sample stacking) were implemented on this easy- handle platform. The linearity of an analytical procedure, as well as the limit of detection (LOD) and the limit of quantification (LOQ) were determined.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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