Naslov
Proinflammatory and remodeling processes in subcutaneous and visceral adipose tissue defined through the activity of immunomodulatory adipocytokines in male children and adolescents

Autori
Mujkić, Robert ; Divković, Dalibor ; Selthofer Relatić, Kristina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, ostalo, znanstveni

Izvornik
4th YOUNG SCIENTISTS’ DAY - BOOK OF ABSTRACTS / Zvonko Antunović - Osijek: Sveučilište Josipa Jurja Strossmayera Fakultet agrobiotehničkih znanosti Osijek, 2022., 11-11 / Antunović Z. - Fakultet agrobiotehničkih znanosti Osijek, 2022, 11-11

ISBN
978-953-8421-01-3

Skup
4th YOUNG SCIENTISTS’ DAY

Mjesto i datum
Osijek, Hrvatska, 07.10.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Recenziran

Ključne riječi
obesity ; early years ; collagen ; inflammation ; macrophages ; adipose tissue

Sažetak
Childhood obesity is a serious public health problem that leads to numerous metabolic diseases which significantly affect quality of life. Aim of this study was to examine the processes affecting remodeling of the extracellular matrix (ECM) in adipose tissue of healthy male children depending on their body weight. Subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples were collected from 75 healthy male subjects who underwent elective orchidopexy or hernia repair. Subjects were divided into two groups depending on their BMI Z-score. Analysis of adipose tissue morphometry was performed by histological staining method hematoxylin and eosin and the assessment of fibrosis in ECM of adipose tissue was performed by Massons's trichrome staining method for collagen detection ; immunohistochemistry was performed for determining the number of CD163+ cells and detection of crown like structures (CLS) ; ELISA was performed for determination of serum adipocytokine levels for - chemerin, visfatin, adiponectin and omentin. rtPCTR was performed for relative gene expression for – IL-6, IL-8, TNF-α and COL6α3 on 20 samples. Overweigth and obese subjects were found to have larger surface area adipocytes in SAT, higher number CD163+ cells in VAT, higher number of CLS in both adipose tissue depots, higher collagen staining in VAT and significantly higher gene expression of COL6α3 in both depots and higher gene expression of IL-8 and IL-6 in SAT. Overweight and obesity was positively correlated with larger surface area in SAT, higher number of adipocytes in VAT, CD163+ cells and CLS in both depots of adipose tissue. In male children, obesity can lead to SAT adipocyte hypertrophy, increase collagen deposition in VAT, increase COL6α3 expression and promote changes in macrophage polarization, affect their accumulation, structural stability of the ECM and the development of inflammation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA