Naslov
Employment of QuEChERS/dSPE extraction procedure for sterigmatocystin (STC) and 5- methoxysterigmatocystin (5-M-STC) from beer and their detection by TLC

Autori
Kožić, Hrvoje ; Šmehil, Ana ; Jakšić, Daniela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arhiv za higijenu rada i toksikologiju- Abstracts of the international symposium Power of Fungi and Mycotoxins in the Midst of Climate Change (PoFMy) / Šegvić Klarić, M. ; Rašić, D. - Zagreb, 2022

Skup
Power of Fungi and Mycotoxins in the Midst of Climate Change

Mjesto i datum
Koprivnica, Hrvatska, 16.09.2022. - 17.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
: chromatographic separation ; exposure ; health effects ; mycotoxins ; toxicity

Sažetak
Sterigmatocystin (STC) and 5- methoxysterigmatocystin (5-M-STC) are structurally related mycotoxins with cytotoxic and genotoxic properties. They are predominantly produced by fungi of the genus Aspergillus, series Versicolores. The presence of these mycotoxins was investigated in indoor environments, while the data regarding food products remains insufficient, especially for 5-M-STC. Given the high consumption of beer in Croatia and the world, the presence of STC and 5-M-STC in beer may negatively affect the health of exposed individuals. Thus, 58 different samples of beer were collected from the Croatian market, extracted by combining the QuEChERS (Quick Easy Cheap Effective Rugged Safe) extraction procedure and dispersive solid phase extraction (dSPE). The prepared extracts were analysed by thin-layer chromatography (TLC) using silica gel 60 as a stationary phase and toulen:ethyl acetate:formic acid 90 vol. % (5:4:1) as a mobile phase. Following chromatographic separation, to enhance the detection of the fluorescent spots assigned to STC and 5-M-STC, the TLC plates were dried and heated for 10 minutes in an oven at 140 °C. Additionally, the TLC plates were sprayed with aluminium chloride solution prepared in ethanol 60 vol. %. The limit of detection (LOD) was 1.3 μg/mL for STC and 3.2 μg/mL for 5-M-STC. While STC was not detected in any of the samples, 5-M-STC was detected in 46.55% of the samples. The presence of 5-M-STC was confirmed by UV-Vis spectroscopy following the extraction of the spot of interest from the TLC plate. Further analysis of the same extracts by application of more sensitive chromatographic techniques will allow for a more accurate interpretation of these observations, allow the detection of STC and 5-M-STC in the concentrations below the LOD, and enable us to quantify both metabolites.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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