Naslov
Metabolic alterations in morbidly obese individuals – a pilot study

Autori
Jaganjac, Morana ; Soldo, Ivo ; Pajtak, Alen ; Vergles, Domagoj ; Soldo, Ana ; Skrzydlewska, Elżbieta ; Zarkovic, Neven

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
18th Annual Conferfence of the Metabolomics Society : Abstract book / - , 2022, 185-185

Skup
18th Annual Conferfence of the Metabolomics Society

Mjesto i datum
Valencia, Španjolska, 19.06.2022. - 23.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Obesity

Sažetak
Obesity is a complex medical condition characterized by an excessive or abnormal fat accumulation and metabolic changes.Moreover, obesity is associated with an increased risk of premature disability and death and with a variety of comorbidities including dementia, depression, metabolic syndrome, type 2 diabetes, cardiometabolic diseases, and cancers.Clinically significant and effective strategies for obese individuals include pharmacotherapy, behavioral therapy and bariatric surgery.This study aimed to identify changes in metabolites in relation to BMI in morbidly obese individuals (BMI≥40) and to study the impact of bariatric surgery on the BMI reduction and metabolic improvement 6 months after the surgery.Plasma samples of clinically well characterized morbidly obese individuals (n=26) undergoing bariatric surgery and healthy controls were collected (n=13), and additionally, several plasma samples were collected post- surgery.Metabolomics analysis of plasma samples was performed on both gas (GC) and liquid (LC) chromatography coupled to mass spectrometer (MS) platforms using in house pipelines for GC-MS and high-resolution LC-MS analysis.The identity of tentative metabolite candidates was confirmed by LC−MS/MS analysis.Our results point to distinct plasma signature of morbid obesity compared to control.Additionally, strong perturbations in the metabolome were observed six months post-surgery indicating specific physiological changes.However, the obtained results remain to be confirmed in a larger cohort in order to understand metabolic perturbations associated with morbid obesity and the impact of bariatric surgery on metabolic improvement.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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