Naslov
Novel Anthracene Analog of Triarylborane Fluorophore with DNA, RNA and Protein Interactions

Autori
Krošl, Ivona ; Ban, Željka ; Griesbeck, Stefanie ; Marder, Todd B. ; Piantanida, Ivo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of 4th Mini Symposium for Young Scientists of the Section of Medicinal and Pharmaceutical Chemistry / - Zagreb, 2020, 8-8

Skup
4th Mini Symposium for Young Scientists of the Section of Medicinal and Pharmaceutical Chemistry

Mjesto i datum
Zagreb, Hrvatska, 08.12.2020

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
triarilborani ; fluorofori ; DNA ; RNA ; protein ; interakcije
(triarylborane ; fluorophore ; DNA ; RNA ; protein ; interactions)

Sažetak
In various biological or biochemical experiments, as well as in many medical diagnostic tests, low molecular weight fluorescent probes are used, with the small size of the molecule presenting a challenge in designing new selective probes. One of our goals is to synthesize a small molecular probe that binds to several different biomacromolecules (DNA, proteins) giving a different spectroscopic response for different biomolecules. We have recently shown that the new triaryl-boron fluorophore (Scheme 1, left) allows simultaneous quantification of DNA / RNA and protein (BSA) in solution, thanks to the fact that it can distinguish between DNA / RNA and BSA by fluorescence emission, Δʎ = 100 nm . In the studies presented here, the newly prepared anthracene analog (Scheme 1, SG252) showed strong binding to ds-DNA, ds-RNA, ss-RNA, and BSA followed by different fluorescent responses. In various biological or biochemical experiments, as well as in many medical diagnostic tests, low molecular weight fluorescent probes are used, with the small size of the molecule presenting a challenge in designing new selective probes. One of our goals is to synthesize a small molecular probe that binds to several different biomacromolecules giving a different spectroscopic response for different biomolecules. We have recently shown that the new triaryl-boron fluorophore (Scheme 1, left) allows simultaneous quantification of DNA / RNA and protein (BSA) in solution, thanks to the fact that it can distinguish between DNA / RNA and BSA by fluorescence emission, Δʎ = 100 nm. In the studies presented here, the newly prepared anthracene analog (Scheme 1, SG252) showed strong binding to ds-DNA, ds-RNA, ss-RNA, and BSA followed by different fluorescent responses.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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