Naslov
Evaluation of activated lymphocytes (RE-LYMP, AS- LYMP) on hematological analyser sysmex XN-1000 with manual method

Autori
Kajinić, Dalibor ; Grdiša, Katarina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book. U: Clinica Chimica Acta, 530(S1) / - , 2022, S175-S175

Skup
24th International Congress of Clinical Chemistry and Laboratory Medicine ; 16th Asia-Pacific Congress of Clinical Biochemistry

Mjesto i datum
Seoul, Republika Koreja, 26.06.2022. - 30.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RE-LYMP ; AS-LYMP

Sažetak
Background-aim Novel hematological parameters for rapid monitoring of the immune system response as reactive lymphocytes (RE-LYMP) and T cell- independent plasma cells (AS-LYMP) obtained from a routine blood count can improve and speed up diagnostic procedure. Reference interval of total reactive lymphocytes are 0-5%, as a % of all white blood cells (WBC). The aim of this study is to evaluate agreement between manual and automated findings of activated lymphocytes. Methods Venous blood (K2E, BD vacutainer) from patients (n=112) whose differential blood count showed total reactive lymphocytes % more than 1 % measured by Sysmex XN1000 were taken for manual differentiation. A medical laboratory scientist counted and described 200 WBC on a stained microscope slide (May-Grunwald-Giemsa staining). Method comparison were assessed in MedCalc statistical program. Results Bland and Altman(B&A) plot of RE-LYMP (measured by Sysmex) and atypical lymphocytes-suspect reactive, suspect neoplastic and plasma cells (counted in blood smear) revealed the BIAS of -0, 3 units (95% CI -1, 0846 to 0, 5614, upper limit 8, 3537 and lower limit -8, 8769) not a constant error but statistically a significant difference. B&A analysis of AS-LYMP (measured by Sysmex) and plasma cells (counted in blood smear) revealed the BIAS of 2, 4 units (95% CI 1, 6488 to 3, 1603, upper limit 7, 2767 and Conclusions The B&A plot method only defines the intervals of agreements. Acceptable limits must be based on clinical necessity, biological considerations and lead to improvement of patient management and help start or modify treatment faster. A microscope will always be gold standard for cell differentiation, but new parameter RE- LYMP in this study showed low BIAS and limits that fit in Westgard and CROQALM defined criteria so we can conclude growing contribution on patient management. AS-LYMP parameter

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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