Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice

Perutina, Ilija; Kelam, Nela; Maglica, Mirko; Racetin, Anita; Ogorevc, Marin; Filipović, Natalija; Katsuyama, Yu; Mišković, Josip; Vukojević, Katarina

doi:10.3390/biomedicines11051321

Pregled bibliografske jedinice broj: 1268446

Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice

Perutina, Ilija; Kelam, Nela; Maglica, Mirko; Racetin, Anita; Ogorevc, Marin; Filipović, Natalija; Katsuyama, Yu; Mišković, Josip; Vukojević, Katarina

Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice // Biomedicines, 11 (2023), 5; 1321, 20 doi:10.3390/biomedicines11051321 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1268446 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice

Autori
Perutina, Ilija ; Kelam, Nela ; Maglica, Mirko ; Racetin, Anita ; Ogorevc, Marin ; Filipović, Natalija ; Katsuyama, Yu ; Mišković, Josip ; Vukojević, Katarina

Izvornik
Biomedicines (2227-9059) 11 (2023), 5; 1321, 20

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
canonical Wnt signaling ; nephrogenesis ; α-tubulin ; inversin ; dishevelled-1 ; Wnt5a/b ; β-catenin ; CAKUT ; Dab1

Sažetak
This study aims to determine the protein expression patterns of acetylated α-tubulin, inversin, dishevelled-1, Wnt5a/b, and β-catenin in developing (E13.5 and E15.5) and early postnatal (P4 and P14) kidneys of Dab1−/− (yotari) mice, their role in regulating the Wnt signaling pathway, and the possible relation to congenital anomalies of kidney and urinary tract (CAKUT). The analysis of target protein co-expression, observed in the renal vesicles/immature glomeruli, ampullae/collecting ducts, convoluted tubules, metanephric mesenchyme of developing kidneys, but proximal convoluted tubules, distal convoluted tubules and glomeruli of postnatal kidneys, was performed using double immunofluorescence and semi-quantitative methods. The overall expression of acetylated α-tubulin and inversin during normal kidney development increases with higher expression in yotari mice as the kidney acquires mature morphology. An increase in β-catenin and cytosolic DVL-1 levels, indicating a switch from non-canonical to canonical Wnt signaling, is found in the postnatal kidney of yotari mice. In contrast, healthy mouse kidney expresses inversin and Wnt5a/b in the postnatal period, thus activating non-canonical Wnt signaling. Target protein expression patterns in kidney development and the early postnatal period observed in this study could indicate that switching between canonical and non-canonical Wnt signaling is crucial for normal nephrogenesis, while the defective Dab1 gene product in yotari mice may promote CAKUT due to interfering with this process.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA

Projekti:
HRZZ-IP-2016-06-2575 - Karakterizacija kandidat gena za kongenitalne anomalije bubrega i urotrakta (CAKUT) tijekom razvoja u miša i čovjeka (CAKUT) (Vukojević, Katarina, HRZZ ) ( CroRIS)

Ustanove:
Medicinski fakultet, Split

Profili:

Katarina Vukojević (autor)