Naslov
Intestinal histomorphology of European seabass fed novel feed formulations

Autori
Hrabar, Jerko ; Bočina, Ivana ; Žuvić, Luka ; Žužul Vrgoč, Iva ; Lepen Pleić, Ivana ; Bušelić, Ivana ; Talijančić, Igor ; Šegvić-Bubić, Tanja ; Grubišić, Leon

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
14th International Aquaculture Conference, Book of Abstracts / Filić, Želimir - : Hrvatska gospodarska komora (HGK), 2023, 116-116

Skup
14th International Aquaculture Conference

Mjesto i datum
Vukovar, Hrvatska, 29.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
poultry by-product meal, insect, Dicentrarchus labrax, intestine, histomorphology

Sažetak
The present work aimed to assess intestinal histomorphology and ultrastructure of seabass fed novel diet formulations. We performed a six- month feeding trial with three groups of seabass using i) commercial (control) diet (CF) ; ii) a control diet high in plant-derived ingredients (CV) ; iii) a CV diet supplemented with 10% insect and 30% poultry by-product protein (VH10P30). At the end of the feeding trial, pieces of the proximal (PI) and distal intestines (DI) from six fish per treatment were processed for histological analysis (HE, AB/PAS). Additionally, intestinal pieces from three fish per treatment were processed for ultrastructural studies. In the CF-fed fish, the proximal intestine showed well-preserved morphology with no degenerative or inflammatory changes. The proximal intestine of the CV-fed fish had irregularly branched villi with extensive enterocyte vacuolisation, detachment of lamina epithelialis from the lamina propria and pronounced inflammatory infiltrate. In VH10P30-fed fish, intestinal villi were irregularly branched with minor enterocyte vacuolisation and focally extensive detachment of l. epithelialis and mild to moderate inflammatory infiltrate. Less pronounced changes were observed in the distal intestine and were mostly limited to the appearance of villi. In conclusion, we confirmed the importance of animal proteins in the seabass diet, as the addition of insect and poultry by-product-derived proteins significantly improved intestinal morphology.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA