Naslov
Cadmium accumulation and biomarker responses in the digestive glands of freshwater mussel Unio crassus following cadmium exposure

Autori
Kiralj, Zoran ; Dragun, Zrinka ; Bušić, Bruno ; Lajtner, Jasna ; Trgovčić, Krešimira ; Ivanković, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th Symposium on Freshwater Biology with the international participation : Book of abstracts / Mičetić Stanković, Vlatka ; Ivković, Marija ; Matoničkin Kepčija, Renata ; Sertić Perić, Mirela ; Miliša, Marko ; Vilenica, Marina - Zagreb : Hrvatsko udruženje slatkovodnih ekologa (HUSEk), 2023, 46-46

Skup
4th Symposium on Freshwater Biology with the international participation

Mjesto i datum
Zagreb, Hrvatska, 21.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
cytosol ; mussel ; cadmium ; biomarkers ; ICP-MS

Sažetak
Cadmium accumulation above a certain threshold can adversely affect aquatic organisms. To evaluate the effects of Cd exposure on aquatic organisms, we monitored changes in the responses of the molecular biomarkers acetylcholinesterase (AChE), catalase (CAT), glutathione S-transferase (GST), malondialdehyde (MDA), metallothioneins (MT), total glutathione (tGSH), and total proteins (TP) in the digestive glands of Unio crassus mussels. In addition, the changes in concentrations of total and cytosolic Cd, Cu, and Zn in the digestive glands of the mussels were monitored. Mussels were exposed to 50 µg/L of Cd for 7 days. Metals were determined using a high-resolution inductively coupled plasma mass spectrometer, and biomarkers were measured spectrophotometrically. The results showed almost 30-fold higher total and cytosolic cadmium concentrations in the digestive glands of Cd-exposed mussels than in control mussels. Furthermore, the activities of biomarkers AChE and CAT were 50% and 70% lower, respectively, in exposed group than in control group. In addition, tGSH concentrations were 20% higher in exposed group than in control group. Other biomarkers showed similar activity/concentration trends in both studied mussel groups. The lack of MT induction in the presence of high Cd accumulation suggests possible toxic effects due to exceeding cellular inducible/defence capacity. Although our study examined only one concentration and exposure duration, the results suggest that Cd exposure can induce significant changes in metal accumulation and biochemical responses in mussels. Nevertheless, further studies are needed to determine the effects of Cd at different concentrations and exposure times and to clarify the mechanisms underlying toxic effects.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

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