Naslov
SERS efficiency of silver nanospheres and nanostars for label-free detection of G-Quadruplex

Autori
Petrović, Petra ; Miljanić, Snežana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th Faculty of Science PhD Student Symposium - Book of Abstracts / Pavlek, Katarina - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2023, 118-118

ISBN
978-953-6076-01-7

Skup
7th Faculty of Science PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 21.04.2023. - 22.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
G-Quadruplex ; nanospheres ; nanostars ; SERS

Sažetak
G-quadruplexes are non-canonical nucleic acid structures of the guanine-rich sequences. They are composed of the stacked planar G-quartets, consisting of four guanine bases connected to each other by Hoogsteen hydrogen bonds. Owing to their unique structural properties and biological functions, G-quadruplexes have attracted significant attention in both basic and applied research, including drug design and development. Among various methods used to understand the structural dynamics and stability of G- quadruplexes, surface-enhanced Raman scattering (SERS) spectroscopy has been recently applied. SERS spectroscopy is a powerful analytical technique which is based on amplification of scattered radiation during adsorption or chemical binding of molecules to a nanostructured metal surface. The enhancement of the Raman scattering is highly dependent on the size, shape, composition, and arrangement of the metal substrate. For instance, sharp tips and edges of the nanostars can generate a stronger electromagnetic field than the smooth surface of the nanospheres, making them more sensitive for SERS detection of target molecules. In this work, two different SERS substrates were used to study structural forms of human telomeric sequence d[TTAGGG]4 (Tel24), capable of forming anti-parallel G-quadruplex in presence of sodium ions. Silver nanospheres and silver nanostars, both stabilized by citrate ions on their surface, were prepared and characterized. SERS spectra of the unfolded Tel24 sequence and Na+ stabilized G-quadruplex were acquired on silver nanoparticles and the spectral differences, distinctive of the structural forms, analyzed. The efficiency of the used SERS substrates was compared.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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